Expression of AP-1 family members was assessed by microarray in BMDM with or without LPS stimulation as described in Fig. 3A. SD from 4 technical replicates is shown. (B) A subset of JUNB-dependent and JUNB-independent genes was identified by microarray and further classified into primary, secondary, tolerizable and non-tolerizable categories. +, Junbfl/fl BMDM. Δ, JunbΔLyz2 BMDM. (C) Junbfl/fl or JunbΔLyz2 BMDM were treated with LPS, and RNA was harvested at the indicated timepoints. Transcript of Il1b (left panel) or Il12b (right panel) was measured by RT-qPCR. Graphs depict means + SD and are representative of two independent experiments, each with three technical replicates.