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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Immunol. 2014 Dec 3;194(1):177–186. doi: 10.4049/jimmunol.1401595

Figure 4. JUNB affects late transcription of primary and secondary genes. (A).

Figure 4

Expression of AP-1 family members was assessed by microarray in BMDM with or without LPS stimulation as described in Fig. 3A. SD from 4 technical replicates is shown. (B) A subset of JUNB-dependent and JUNB-independent genes was identified by microarray and further classified into primary, secondary, tolerizable and non-tolerizable categories. +, Junbfl/fl BMDM. Δ, JunbΔLyz2 BMDM. (C) Junbfl/fl or JunbΔLyz2 BMDM were treated with LPS, and RNA was harvested at the indicated timepoints. Transcript of Il1b (left panel) or Il12b (right panel) was measured by RT-qPCR. Graphs depict means + SD and are representative of two independent experiments, each with three technical replicates.