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. 2015 Apr 16;106(3):509–519. doi: 10.1093/cvr/cvv131

Figure 4.

Figure 4

PKCε activation results in Nrf2 nuclear translocation. (A) HUVECs were transfected with CA-PKCε or Ad0 (MOI 100) for 16 h prior to staining with an anti-Nrf2 antibody and DRAQ-5 nuclear stain. Representative images are shown, and in (B) a histogram of pooled nuclear fluorescence data from five individual experiments, expressed as MFI of Nrf2 (mean ± SEM). (C) HUVECs were left UT or transfected with CA-PKCε or Ad0 (MOI 100) for 16 h. Nuclear lysates were generated and Nrf2 activation assessed using a DNA-binding ELISA in the presence and absence of a competitive WT oligonucleotide (WT), corresponding to the consensus sequence for Nrf2 binding. Pooled data (mean ± SEM) from at least four independent experiments are shown, corrected for tubulin expression where appropriate, and normalized to Ad0-transfected control cells (t-test, *P < 0.05).