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. Author manuscript; available in PMC: 2016 May 21.
Published in final edited form as: Chem Soc Rev. 2015 Mar 17;44(10):3036–3055. doi: 10.1039/c5cs00020c

Figure 9.

Figure 9

Molecular beacons serving as signal reporter for single nucleotide polymorphism detection. (A) A reverse MB (rMB) is formed by the complementary arm sequences of the ligated primers. Real-time single-pair fluorescence resonance energy transfer (spFRET) measurements were performed in a poly (methyl methacrylate) (PMMA) microfluidic device to detect rMBs formed in a ligation detection reaction (LDR) assay where point mutations in K-ras codon 12 were detected. (B) Design of a molecular beacon-based junction probe system for amplified single nucleotide polymorphism detection. Reprinted with permission from ref. 107. Copyright (2010) American Chemical Society.