Skip to main content
. Author manuscript; available in PMC: 2015 May 14.
Published in final edited form as: Front Biosci (Landmark Ed). 2011 Jun 1;16:2598–2621. doi: 10.2741/3875

Figure 4.

Figure 4

Bone sialoprotein is immunoprecipitated together with 60 kDa cationic type XI N-terminal fragment only from mineralized osteoblastic cultures. Extracts of mineralized and un-mineralized osteoblastic cultures were immunoprecipitated with anti-bone sialoprotein antibodies. Immunoprecipitates were subjected to SDS-PAGE and the same gel was stained sequentially with Stains All (left) and then with Coomassie blue dye (right). KEY: +BGP +AEBSF, extract from culture where mineralization was inhibited by 4-(2-aminoethyl)-benzenesulfonyl fluoride hydrochloride; +BGP, extract from mineralized culture; marker, molecular weight standards.