Table 4. Aedes aegypti L4 mortality rates after incubation with fractions (F1, F2, and F3) obtained after the separation of secondary metabolites from Schinus terebinthifolius leaf extract.
| Parameter | Sample | |||
|---|---|---|---|---|
| F1 | F2 | F3 | Control | |
| Mortality rate (%) | ||||
| 24 hours | 0.0 a | 10.0 ± 0.0 b | 0.0 a | 0.0 a |
| 3 days | 100.0 a | 33.3 ± 5.7 b | 6.7 ± 2.8 c | 3.3 ± 2.8 c |
| 8 days | 100.0 a | 66.7 ± 2.8 b | 6.7 ± 2.8 c | 3.3 ± 2.8 c |
| Pupation (%) | 0.0 a | 0.0 a | 11.6 ± 5.7 b | 10.0 ± 0.0 b |
| Adult emergence (%) | 0.0 a | 0.0 a | 58.3 ± 5.7 b | 68.3 ± 7.6 b |
| Larvae that eliminated gut content (%) | 30.0 ± 5.0 a | 0.0 b | 0.0 b | 0.0 b |
F1: fraction eluted with Tris buffer, containing cinnamic acid derivatives. F2: fraction eluted with methanol, containing flavonoids and traces of cinnamic acid derivatives. F3: fraction eluted with methanol-acetic acid, containing hydrolysable tannins. Elimination of gut content was evaluated with 24 h of experiment. Pupation and adult emergence rates were calculated 8 days after larval incubation. Different letters at the same line indicate significant differences (p<0.05) between the treatments.