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. 2015 May 14;11(5):e1005233. doi: 10.1371/journal.pgen.1005233

Fig 1. Identification of 4E-BP associating proteins using TAP-tag IPs.

Fig 1

A. Western blots of the TAP IPs, lanes 1–9 show input, flowthrough and eluates for the IP strains and lanes 10–13 show the eluates of the 4E-BPs with and without RNase I digestion. Blots were probed with the α Protein A peroxidase (PAP) antibody (detects the TAP-tag), αCaf20p, αeIF4E, αeIF4G and αRps3p to highlight IP efficiency and maintenance of previously published associations. B. Proportional Venn-style diagram depicting the overlaps between proteins associating with the 4E-BPs. C. Gene Ontology classes statistically overrepresented within 4E-BP associated proteins at indicated FDR significance. Only a representative selection of the significant functional categories is shown D. Western blots from reciprocal TAP IPs of a selection of RNA-binding proteins identified as interacting with the 4E-BPs probed with the indicated antibodies. E. Effect of RNase I digestion on protein interactions identified in D.