Fig 5. 4E-BP-bound mRNAs enrich cell cycle and transcription factors.

A. Functional GO class analysis of mRNAs associated with the 4E-BPs by RNA-seq. Over/underrepresentation colours depict FDR significance as per the key. Only a representative selection of the significant functional categories is shown. B. Summary of morphology changes noted within the Saccharomyces cerevisiae Morphological database (SCMD; http://yeast.gi.k.u-tokyo.ac.jp/datamine/) for 4E-BP deletion strains. Red/blue shading indicate over/under enrichment compared to wild type cells. C. D. Cell cycle progression defects in caf20 mutants. Flow cytometry analysis of DNA content using Sytox Green staining is shown at the indicated times (min) following release from alpha factor arrest for C. wild type and mutated cells, and D. plasmid complemented mutant cells.