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Box-plots showing step-based migration angles calculated from 2P microscopic observation of PKCθ-/- (KO) and wild-type (WT) T cells: A) Migration angles for KO and WT T cells without regard to dyes used in observations; B) The same data broken out by cell type and the two dyes used to image them, CFSE and CMTMR, illustrating the confounding effect of the dyes on T cell migration angles.
