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. 2015 May 14;10(5):e0127239. doi: 10.1371/journal.pone.0127239

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© 2015 Kan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) Western blot analysis of p65shRNA- HONE1 cells showed reduced levels of phosphorylated p65 S536, total p65, and total IκBα compared to scramble control. IκBα-SR- HONE1 cells only showed a decreased amount of phosphorylated p65 S536, but did not affect the overall amount of total p65 when compared to pWPI-VA. Endogenous and exogenous IκBα are labeled as shown. The p84 was used as a loading control for scramble and p65 shRNA, pWPI-VA and IκBα-SR, separately. Protein expression of N-cadherin and total EGFR were all reduced in p65 shRNA and IκBα-SR HONE1 cells compared to scramble and pWPI-VA control. The (^) indicates the exogenous 3xHA-IκBα-SR. (B) Western blot analysis of p65 WT-overexpressing HONE1 cells showed increased acetylation at K310 and enhanced phosphorylation at S536 compared to VA cells. The p65 WT overexpression increases the protein levels of IκBα, snail, slug, twist, N-cadherin, and sox9, compared to VA cells. The p84 was used as a loading control. The (✚) indicates the exogenous RFP-p65 WT. The (★) indicates the acetylation band in the exogenously expressed RFP-p65 WT.