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. 2015 May 14;10(5):e0127348. doi: 10.1371/journal.pone.0127348

Fig 1. General characteristics of tumor spheres formed from multiple cell lines.

Fig 1

A. Significant heterogeneity existed in the size of TS as shown by representative pictures of TS from H1299 and MCF7 cultured in TS medium for 8 days. Counting of smaller TS (solid arrow) was sometimes prone to subjectiveness. TS derived from certain cell lines (HCT116) might have strong tendency to fuse (hollow arrow). B. “size of spheres” for H1299 TS showed a skewed distribution with smaller spheres account for the majority of population. H1299 cells were plated in 24-well ULA plate (2000 per well in 2ml of TS medium) to allow TS formation for 4 days before microscopic assessment. The results were presented as Mean ± SEM from 6 replicates. C. Some tumor cell lines formed typical 3-dimensional spherical structures with sharp edges (A), while others were not, or require special supplement (hollow arrow).