Skip to main content
. 2015 May 14;11(5):e1004836. doi: 10.1371/journal.ppat.1004836

Fig 2. Circulating pore-forming PLY mediates cardiac injury and inflammation in vivo.

Fig 2

(A) Representative Western blots showing circulating cTnI and cTnT in murine plasma following i.v. injection of PLY (200 ng/g) and PdB (400 ng/g). (B) Mean±SD of circulating cTnI from 4 mice (each group) that survived at 24 h after treatment with PLY and PdB. *p<0.05 as compared to PdB group. (C) Survival curves of mice injected (i.v.) with PLY (400 and 200 ng/g) or PdB (400 ng/g) (n = 8 each group). (D) Pathological examination of murine heart sections after infection with purified PLY (200 ng/g) (a, b and d) and PdB (400 ng/g) (c). H&E representative images (a-c) of murine heart sections under x60 magnification showing inflammatory cell infiltrations (arrows) in mice dying within 6 hours (a) and those surviving for 24 hours (b). (c) PdB injection causes no pathological abnormality in murine myocardium. (d) Absence of active caspase-3 staining in murine myocardium following PLY injection.