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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Ann Surg. 2015 Feb;261(2):368–377. doi: 10.1097/SLA.0000000000000635

Figure 4. Disrupting N-cadherin interactions increases Endothelial Cell Permeability.

Figure 4

N-cadherin expressing HUVEC endothelial cells were grown to confluence in transwell plates and tested for changes in permeability in response to ADH-1 or VEGF. In the presence and absence of serum and GF, pretreatment of endothelial cells (HUVEC) with ADH-1 nearly doubled their permeability to a 40kDa FITC-conjugated dextran compared to saline controls. This increase in permeability in response to ADH-1 even exceeded the effects of VEGF, used as a positive control for changes in endothelial cell permeability. N-cadherin expression, normalized to GAPDH, in HUVEC cells is shown with respect to N-cadherin expression in the melanoma cell line DM366, a high N-cadherin expressing melanoma cell line.