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. Author manuscript; available in PMC: 2016 May 13.
Published in final edited form as: Metallomics. 2015 Mar 13;7(5):847–856. doi: 10.1039/c5mt00052a

Fig. 4. Overexpression of fpn-1.1 in pdr-1 mutants improves mitochondrial integrity and antioxidant response.

Fig. 4

(A) Relative mitochondrial DNA (mtDNA) copy number in pdr-1 KO and pdr-1 KO; fpn-1.1 OVR animals following an acute, 30 min treatment with 0 and 5 mM MnCl2. Relative gene expression was determined by qPCR. (B) Two-way ANOVA analysis of data in 4A showing genotype significance in mtDNA copy number. (C) Total glutathione (GSH) levels of pdr-1 KO and pdr-1 KO; fpn-1.1 OVR animals following an acute, 30 min treatment with 0 and 5 mM MnCl2. (A) Relative mtDNA copy number is expressed as a ratio of nd-1 (mtDNA marker) to cox-4 (nuclear DNA marker). Data are expressed as mean values + SEM of at least five independent experiments in duplicates normalized to the untreated N2 wildtype values. (C) Data are expressed as mean values + SEM of at least five independent experiments in duplicates, normalized to total protein content and relative to untreated pdr-1 KO values. Statistical analysis by two-way ANOVA: (A) interaction, ns; genotype, p=0.0116; concentration, ns; (B) interaction, ns; genotype, ns (trend level, p=0.09); concentration, ns.