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. Author manuscript; available in PMC: 2016 May 13.
Published in final edited form as: Nano Lett. 2015 Apr 7;15(5):2765–2772. doi: 10.1021/nl502227a

Figure 4. Functional analysis of hiPSC-derived cardiomyocyte spheroids.

Figure 4

(A) Average contraction amplitude from Day 1 to Day 7 of each group. Double asterisk (**) represents statistical difference between all groups. (B, C) Representative fractional area change (i.e., contraction amplitude) of spontaneously beating spheroids with and without e-SiNWs at time points Day 0 and Day 6; n = 6 spheroids per condition. (D, E) Representative calcium transient imaging of 7 regions of interest (colored circles) per spheroid for each group. Fluorescence amplitude, F/F0, refers to measured fluorescence intensity normalized to background fluorescence intensity. (F) Comparison of the peak value of F/F0 for each group. (n=3) (G) Comparison of calcium release duration for each group. (n=3) hiPSC-NC= human induced pluripotent stem cell cardiac spheroids, no e-SiNWs, no stimulation; hiPSC-WC= human induced pluripotent stem cell cardiac spheroids, with e-SiNWs, no stimulation. Asterisks (*) represent statistical significance with p<0.05; error bar represents standard deviation. Scale bars = 100 μm.