TABLE 1.

Fungal culture, histopathology, and PCR results for the case patient^a

Site	PDOI and wound cultureb	PDOI and histopathology (specific morphology, if available)		PDOI and PCR result (specimen)
		Vascular invasion	No vascular invasion
Left lower extremity	2 (single sample)—yeast, Aspergillus species, Geotrichum species, Alternaria species
Left triceps	18—P. aphanidermatum; 14—P. aphanidermatum (UTHSCSCA DI14-349)	18—fungal elements	23—medium caliber fungal hyphae with inconspicuous branchings; 22—medium caliber fungal hyphae with branchings and a few spores; 20—superficial fungal elements	42—C. elegans (FFPE autopsy); 14—P. aphanidermatum (culture) (UTHSCSCA DI14-349)
Left gluteus	16—P. aphanidermatum; 15—P. aphanidermatum	15—predominantly septate fungal elements	33—medium to broad nonseptate hyphae with direct angular branchings
Left acetabular tissue	17—P. aphanidermatum	19—fungal elements; 17—fungal elements; 15—fungal elements; 14—fungal elements	18—Fungal elements in muscle	17—S. vasiformis (culture)
Small bowel	18—P. aphanidermatum	18—no fungal elements identified
Right leg tissue	18—P. aphanidermatum	32—broad nonseptate and direct angular hyphae	18—fungal elements with and without vascular invasion	18—C. elegans (culture)
		15; fungal elements
Right posterior thigh	25—P. aphanidermatum	28—broad nonseptate and ribbon-shaped fungal elements with direct angular hyphae	25—deep colonization of fungus. Broad nonseptate hyphae with direct angular branchings
	18—P. aphanidermatum	20—focal fungal vascular invasion; 19—focal fungal vascular invasion; 18—focal fungal vascular invasion	14—broad nonseptate hyphae; 12—broad nonseptate fungal forms
Right gluteus maximus		32—broad nonseptate and ribbon-shaped fungal elements with direct angular hyphae
Right hip disarticulation	29—P. aphanidermatum; 25—P. aphanidermatum	32—broad nonseptate and ribbon-shaped fungal elements with direct angular hyphae
Sacrum		35—broad nonseptate and ribbon-shaped fungal elements with direct angular hyphae
Brain		42—fungal elements (autopsy)		42—L. corymbifera (FFPE autopsy)
Testis		42—fungal elements (autopsy)

^aPDOI, day after date of injury.

^bSpecimen identification of Pythium aphanidermatum was confirmed via PCR on culture from PDOI 14 only; all other “culture positives” were identified via in vitro morphology similar to that of this specimen.