. 2015 May 14;53(6):1915–1920. doi: 10.1128/JCM.00358-15

TABLE 1.

Comparative results of rRT-PCR versus sequencing analysis of partial VP1 and 5′-UTR of enterovirus genome

rRT-PCR result	No. of specimens with indicated result of sequencing analysis of VP1 and 5′-UTR		Total
rRT-PCR result	EV-D68^a	Non-EV-D68^b	Total
Positive	71	3^c	74
Negative	1	119	120
Total	72	122	194

Sensitivity, 98.6%.

Specificity, 97.5%.

For these 3 NP specimens, a mixed infection of EV-D68 and HRV was confirmed by additional DNA sequencing analysis of partial VP1 sequences based on EV-D68-specific PCR primers (see the text for more detail). The adjusted diagnostic specificity of rRT-PCR was 100%.