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. 2015 May 14;59(6):3290–3297. doi: 10.1128/AAC.00310-15

TABLE 2.

Summary of the genotypic and phenotypic characteristics of organisms harboring oqxAB-like elements

Bacterial species and test population/specific straina Place/yr(s) of isolation Location of oqxABb Nucleotide sequence homology with pOLA52 (%) oqxAB genotyping with 5 primer sets (no. [%] or present [+] or absent [−])
 MIC (mg/liter)c
oqxA oqxB HAE oqxAB2 oqxAB4 Overall CIP NAL NOR CHL OLA
K. pneumoniae
    HK (n = 85) HK/1984–2011 67 35 32 83 73 85 (100)
    SGSC (n = 8) SGSC/1996 8 4 8 8 8 8 (100)
    MGH 78578 Chromosome 99 + − + + + + 1 ≥128 4 ≥128 16
    QE319 HK/1984 Chromosome 99 − − + + − + <0.006 <4 <2 <4 <16
    94-3d HK/1994 Chromosome NDe + − − + − + 0.25 ≥128 ≤2 64 512
    GN53d HK/2006 Chromosome ND + − + + + + 4 ≥128 ≥64 ≥128 512
    06-2 HK/2006 Chromosome ND + − − + + + ≤0.006 ≤4 ≤2 ≤4 ≤16
K. oxytoca (SGSC, n = 8) SGSC/1996 88f 0 0 4 8 8 8 (100) <0.012–0.05 <4–>128 <4 <4 8–32
E. aerogenes (SGSC, n = 15) SGSC/1996–1999 87f 0 10 15 15 4 15 (100) <0.012 <4–8 <4 <4 8–64
E. cloacae (SGSC, n = 27) SGSC/1996–2000 87f 3 15 9 14 15 26 (96) <0.012–0.05 <4–16 <4 <4–16 16–128
S. Typhimurium (n = 17) HK/2007 5 5 3 4 4 5 (29)
    1792 HK/2007 + + − − + + 2 ≥128 ND ≥128 ≥512
    2005 HK/2007 Plasmid 100 + + − − + + 2 ≥128 ND ≥128 ≥512
Other speciesg HK/2006–2010 Plasmid 100
a

HK, Hong Kong; SGSC, Salmonella Genetic Stock Centre, Calgary, Canada.

b

Based on the results of S1-PFGE and Southern hybridization studies.

c

CIP, ciprofloxacin; NAL, nalidixic acid; NOR, norfloxacin; CHL, chloramphenicol; OLA, olaquindox.

d

K. pneumoniae strains 94-3 and GN53 exhibited the typical oqxAB-mediated antibiotic resistance profile (resistance to olaquindox, chloramphenicol, and the quinolones) without an oqxAB-harboring plasmid.

e

ND, not determined.

f

Based on the nucleotide sequences of the standard strains KCTC 1686, KCTC 2190, and ATCC 13047 for K. oxytoca, E. aerogenes, and E. cloacae, respectively.

g

All other bacterial species tested, including Serratia spp. (n = 7), Pseudomonas aeruginosa (n = 30), Acinetobacter spp. (n = 30), Vibrio parahaemolyticus (n = 30), other Vibrio spp. (n = 30), Staphylococcus aureus (n = 30), and Enterococcus spp. (n = 30), were oqxAB negative.