TABLE 1.

Comparison of conidial versus fragmented-mycelium inoculum for CLSI method using a set of clinical isolates

Clinical strain	AFAa	nb	Concn range (mg/liter) for method:
			Conidiumc		Fragmented-mycelium inoculum prepn methodd
			MIC	MEC	MIC	MEC
Trichophyton mentagrophytes	AMP	4	0.25–1.0		1.0
	TER	4	0.03		0.03–0.06
	FLC	4	16.0–32.0		16.0–32.0
	POS	4	0.5–1.0		0.25–2.0
	VRC	4	0.125–0.5		0.5
	ITR	4	0.25–2.0		1.0
	MCA	4		0.016		0.016–16.0
	ANI	4		0.016–0.03		0.016–16.0
	CAS	4		0.25–1.0		0.06–0.5
Trichophyton tonsurans	AMP	3	0.06–0.5		0.25–1.0
	TER	3	0.03		0.03
	FLC	3	0.5–32.0		8.0–32.0
	POS	3	0.5–1.0		0.25–0.5
	VRC	3	0.06–0.5		0.03–0.25
	ITR	3	0.125–1.0		0.125–2.0
	MCA	3		0.016–0.125		0.016–0.03
	ANI	3		0.016–0.03		0.016–0.03
	CAS	3		0.25–2.0		0.06–1.0
Trichophyton rubrum	AMP	7	0.125–0.5		0.5
	TER	7	0.03		0.03
	FLC	7	0.125–32.0		0.25–4.0
	POS	7	0.06–1.0		0.06–0.5
	VRC	7	0.03–2.0		0.03–0.06
	ITR	7	0.06–1.0		0.06–1.0
	MCA	7		0.016		0.016
	ANI	7		0.016		0.016
	CAS	7		0.125–1.0		0.5–1.0
Microsporum canis	AMP	4	0.06–0.25		0.25
	TER	4	0.06		0.03–0.06
	FLC	4	4.0–16.0		2.0–32.0
	POS	4	1.0		0.5
	VRC	4	0.06–0.125		0.03–0.125
	ITR	4	0.25–1.0		0.125–1.0
	MCA	4		0.016		0.016
	ANI	4		0.016		0.016
	CAS	4		0.25–0.5		0.125–0.5

^aTested antifungal agents (AFAs): amphotericin B (AMP), terbinafine (TER), fluconazole (FLC), posaconazole (POS), voriconazole (VRC), itraconazole (ITR), micafungin (MCA), anidulafungin (ANI), and caspofungin (CAS).

^bNumber of isolates; each isolate was tested once.

^cMicrodilution testing according to Clinical and Laboratory Standards Institute (CLSI) method (6) using conidia for inoculum preparation.

^dMicrodilution testing according to Clinical and Laboratory Standards Institute (CLSI) method using fragmented mycelium for inoculum preparation.