TABLE 4.

MICs for key Gram-negative bacteria with systematic changes in permeability and efflux

Species	MIC (μg/ml)a								Source or reference
	KBD	RIF	NOVO	CAM	ERY	IMI	CIPRO	TET
E. coli BW25113 wild type	>128	16	>128	32	128	0.25	0.016	4	E. coli Genetic Stock Center
E. coli BW25113 ΔtolC	32	16	4	8	4	0.5	0.004	1	This study
E. coli BW25113 ΔtolC DmukB::kan	4	4	0.5	1	2	0.125	0.004	0.5	This study
E. coli BW25113 ΔtolC DmukF::kan	16	8	1	2	4	0.5	0.008	1	This study
E. coli MB4903 wild type	>128	16	>128	16	128	0.25	0.031	>128	29
E. coli MB4902 lpxC101	>128	0.0625	64	4	2	0.063	0.008	64	29
E. coli MB5747 tolC::Tn10	128	8	2	2	4	0.5	0.004	64	30
E. coli MB5746 tolC::Tn10 lpxC101	32	0.5	2	2	0.25	0.5	0.156	128	30
P. aeruginosa MB5919 (CB0046) wild type	32	32	>128	>128	128	8	1	32	31
P. aeruginosa MB5890 (CB1101) Efflux mutantb	64	32	128	2	16	1	0.004	0.5	This study
P. aeruginosa ATCC 12055 wild type	16	8	>128	128	128	1	0.125	16	ATCC
P. aeruginosa ATCC 35151 hypersusceptible	1	0.5	2	4	2	0.25	0.063	0.5	ATCC
A. baumannii ATCC 19606 wild type	16	2	8	128	64	0.25	1	4	ATCC
A. baumannii ATCC 19606 ΔlpxC	0.031	0.0005	0.625	16	0.25	0.063	0.25	0.5	This study
A. baumannii NCIMB12457 wild type	32	4	16	>128	32	0.5	0.5	8	Microbiologics Inc.
A. baumannii NCIMB12457 ΔlpxA	0.125	0.0005	0.063	64	0.25	0.063	0.25	0.5	This study

^aMICs were determined by the CLSI broth microdilution method. Abbreviations: KBD, kibdelomycin; RIF, rifampin; NOVO, novobiocin; CAM, chloramphenicol; ERY, erythromycin; IMI, imipenem; CIPRO, ciprofloxacin; TET, tetracycline.

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