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Enhancement in bacterial fitness increases antibiotic resistance levels in plasmid-bearing clones. Rd populations propagated for 20 days were transformed with the original plasmid pB1000 (Rd-20/pB1000). (A) Relative fitnesses (average ± SEM) of the original Rd/pB1000 (light gray bars) and Rd-20/pB1000 clones (one clone per population) (dark gray bars) compared to that of the plasmid-free ancestor H. influenzae Rd KW20. (B) MICs (average ± SEM) of ampicillin for the same clones.
