TABLE 1.
Calculated EC50, EC90, CC50, CC90, SI50, and SI90 values
| Assay | Cell density (cells/cm2) | EC50 (μM) (95% CI)b | EC90 (μM) (95% CI)b | ||
|---|---|---|---|---|---|
| icBKPyVa | 10,000 | 0.20 (0.16–0.24) | 0.46 (0.33–0.58) | ||
| 20,000 | 0.28 (0.26–0.30) | 0.59 (0.55–0.62) | |||
| 40,000 | 0.37 (0.33–0.41) | 0.80 (0.75–0.86) | |||
| Pooled | 0.27 (0.24–0.29) | 0.59 (0.53–0.65) | |||
| CC50 (μM) (95% CI)b | CC90 (μM) (95% CI)b | SI50 (CI)c | SI90 (CI)c | ||
| BrdU | 20,000 | 0.2 (0.1–0.3) | 1.3 (1.1–1.5) | 0.8 (0.5–1.2) | 2.2 (1.8–2.7) |
| 40,000 | 1.7 (1.3–2.0) | 2.8 (2.1–3.5) | 4.5 (3.2–6.1) | 3.5 (2.4–4.7) | |
| Resazurin | 20,000 | 1.1 (0.6–1.6) | 5.4 (3.5–7.4) | 4.0 (1.9–6.2) | 9.3 (5.7–13.3) |
| 40,000 | 5.1 (4.6–5.6) | 9.3 (8.2–10.5) | 13.8 (11.2–17.1) | 11.6 (9.5–14.0) | |
| Total ATP | 10,000 | 1.3 (1.1–1.6) | 3.4 (2.6–4.1) | 6.7 (4.5–10.1) | 7.4 (4.5–12.4) |
| 20,000 | 2.4 (1.8–3.1) | 5.2 (3.5–6.9) | 8.8 (6.1–11.8) | 8.8 (5.6–12.4) | |
| 40,000 | 3.9 (3.4–4.3) | 6.8 (5.8–7.7) | 10.4 (8.3–13.0) | 8.4 (6.8–10.3) | |
| Membrane integrity | 10,000 | 5.6 (5.4–5.8) | 8.1 (7.4–8.9) | 28.4 (22.5–37.2) | 17.9 (12.9–26.5) |
| 20,000 | 7.9 (7.6–8.3) | 11.1 (10.6–11.6) | 28.5 (25.7–31.6) | 18.9 (17.0–20.9) | |
| 40,000 | 7.8 (7.3–8.2) | 10.9 (10.2–11.5) | 21.0 (17.7–25.0) | 13.5 (11.8–15.4) |
a
icBKPyV refers to the number of intracellular BKPyV genome equivalents per cell.
b
95% confidence intervals were calculated using the curve-fitting software XLfit with a 4-parameter logistic model (model 208).
c
Confidence intervals for SI90 and SI50 values were calculated as follows: lower limit = (CC90 − 95% CI)/(EC90 + 95% CI); upper limit = (CC90 + 95% CI)/(EC90 − 95%CI).