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. 2015 May 14;59(6):3098–3108. doi: 10.1128/AAC.04858-14

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Copyright © 2015, Baylay et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.

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FIG 5 — Measurement of activity of the patA promoter with and without the tRNA gene found upstream of patA copy 2 in strains containing the duplication. (A) Representation of the cloning of the tRNA gene and patA promoter upstream of a promoterless gfp gene in vector pBAV1K2. (B) Fluorescence levels measured from R6 cells containing pBAV1K2 (Empty vector), pBAV1K2p (p), and pBAV1K2tp (tp). Error bars represent the standard deviations from three biological replicates. *, fluorescence significantly greater than that from pBAV1K2p (P < 0.05, one-tailed Student's t test).