TABLE 2.

Details of PCR primers used in this study

Primer pair	Forward primer sequence	Reverse primer sequence	Amplicon
1	CTTATTGGTGGGGAGAAGAA	GATAACGCGGTTGCAGAAGT	Duplication junction
2	GATAGGGCAGAAGAGCATCC	GATAACGCGGTTGCAGAAGT	Intergenic region between hexA and patA
3	TCTTGCTCAGTCCATCATCGAATAT	CAGCATCGGTTCCTTGTC	patA copy 1
4	CTTATTGGTGGGGAGAAGAA	CAGCATCGGTTCCTTGTC	patA copy 2
5	ATGTTGTCCTCGCAGCCTAT	CAGCATCGGTTCCTTGTC	patA internal region containing magellan2 insertion
6	TAGTATCGACGGAGCCGATT	GCTAGTTGAACGCTTCCATC	Cloning site of plasmid pBAV1K2
7	TAAGAATTCTTTAATGAGCGATAGAAGAGTCAG	GATAACGCGGTTGCAGAAGT	Duplication junction, adding a 5′ EcoRI site (underlined sequence)