TABLE 2.
Genotypic ESBL characteristics of nWT isolates in fecal samples collected monthlya
| Dog | ESBL(s) detected (no. of isolates) at mo: |
|||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | |
| 1A | CTX-M-1 (2), CTX-M-14 | X | X | X | ||
| 1B | NE (2) | CTX-M-15 (3) | X | X | ||
| 2A | X | X | CMY-2 | NE (1) | CTX-M-3, NE (1) | X |
| 3A | X | X | X | NE (1) | X | X |
| 4A | X | X | X | X | X | |
| 5A | X | X | X | X | X | X |
| 5B | X | NE (1) | CTX-M-15 (3) | X | X | CTX-M-15 (3) |
| 6A | X | X | NE (1) | X | X | CTX-M-1 |
| 6B | X | X | X | X | X | CTX-M-1, CTX-M-14 (2), SHV-12, TEM-1b and -52StPaul |
| 7A | X | CTX-M-1 (5) | X | NE (2) | ||
| 7B | X | NE (2) | X | CMY-2 (2) | ||
| 7C | X | X | X | X | ||
| 8A | X | X | X | X | ||
| 9A | CTX-M-15, CTX-M-14, CMY-2, NE (1) | CTX-M-2 (3) | CTX-M-1 (3) | CTX-M-1, CTX-M-15, CTX-M-14 | SHV-12 (3) | NE (3) |
| 9B | NE (3) | CTX-M-2 (3) | CTX-M-1 (2), SHV-12 | X | X | CTX-M-1 (3) |
| 9C | CTX-M-15 (2), NE (2) | CTX-M-2 (3) | CMY-2 (3) | CTX-M-1 (2), CTX-M-3 | SHV-12 (3) | CTX-M-1 |
| 10A | CTX-M-1, NE (2) | CTX-M-1 (3), TEM-1b and -52c | CTX-M-15, CMY-2 (2) | CTX-M-15 (3) | SHV-2, SHV-12, TEM-1b and -52StPaul | |
| 11A | CTX-M-1, TEM-52c, NE (1) | CTX-M-1, CTX-M-14, NE (1) | CTX-M-1 (3) | CTX-M-14 (2), NE (1) | CTX-M-1, CTX-M-2, CTX-M-65 | CTX-M-1, CTX-M-15 (2) |
| 12A | X | CMY-2, NE (3) | CTX-M-1 (3) | X | X | X |
| 12B | X | NE (3) | X | X | X | X |
| 13A | X | X | X | X | X | |
| 14A | X | X | X | X | X | |
| 15A | CTX-M-1 (2), NE (1) | CTX-M-32, CTX-M-14, TEM-52c, CMY-2 | CTX-M-1 (4) | CTX-M-1 (2), SHV-12 (2) | CTX-M-1, CMY-2, CMY-134, NE (1) | CTX-M-15 (3) |
| 15B | TEM-1b and -52c (3) | X | X | CTX-M-1 | ||
| 15C | CTX-M-1, CTX-M-14, CMY-2, TEM-1b and -52c | NE (3) | CTX-M-1 (2), SHV-12, CMY-2 | CTX-M-1 (2), NE (2) | CTX-M-1, CTX-M-15 (2) | TEM-52StPaul |
| 16A | CTX-M-1, CMY-2 | X | NE (1) | CTX-M-2 (2), CTX-M-14, TEM-52c | ||
| 16B | CTX-M-1 (3) | CMY-2 (3) | X | CTX-M-32 | ||
| 17A | NE (3) | CTX-M-32 (3) | SHV-12 | CTX-M-1 (3) | X | CTX-M-14, NE (2) |
| 17B | NE (3) | CTX-M-1 (2), CTX-M-14 | X | CTX-M-1, NE (2) | CTX-M-8 (2), CTX-M-14, TEM-52c | CTX-M-14, NE (2) |
| 18A | CTX-M-15 (2), NE (1) | CTX-M-1 (2), NE (2) | CTX-M-1 (3) | CTX-M-1 (2), CTX-M-15 | CTX-M-1 (2), CTX-M-32 | CMY-2 (2), TEM-52StPaul |
| 19A | CMY-2 (2), TEM-1b and -52StPaul | SHV-12 (2), CMY-2 | CTX-M-1 (2), CTX-M-55, CTX-M-14 | CTX-M-1 (2), CTX-M-15 | NE (1) | CTX-M-1 (3) |
| 20A | X | X | X | X | X | X |
| 21A | X | CTX-M-1, SHV-12 (2) | CTX-M-1 | X | X | X |
| 21B | CTX-M-1, CTX-M-14, SHV-12 (2) | CTX-M-1, SHV-12 (2) | CMY-2, NE (1) | X | NE (1) | X |
| 22A | SHV-12, CTX-M-55 (2) | CTX-M-1, CTX-M-2, TEM-1b and -52c, NE (1) | CTX-M-32, CTX-M-2, TEM-52c | CTX-M-1 (2), CTX-M-2 | NE (2), CTX-M-1 | CTX-M-1, CTX-M-65, NE (1) |
| 23A | NE (2) | CTX-M-1, NE (2) | CTX-M-1 (2), TEM-52c and -135 (2) | X | ||
| 24A | NE (3) | CMY-2, TEM-52c (2) | CMY-2 (2), TEM-52c, SHV-12 | CTX-M-1 (3) | CTX-M-1, SHV-12, TEM-52c, NE (1) | X |
| 24B | NE (3) | CTX-M-1, CMY-2, NE (1) | CMY-2 | CTX-M-1 (3) | SHV-12, TEM-52c (3) | X |
X, fecal sample without growth on MCC; NE, ESBL-negative isolates with non-wild-type susceptibility to cefotaxime, including non-Enterobacteriaceae. Dogs with the same numbers shared the same household. If a fecal sample produced >1 isolate containing similar genes, the number of isolates is shown in parentheses. Of isolates with combinations of resistance genes, only ESBL-type genes are shown. The CTX-M-1/61 gene type is shown as CTX-M-1, CTX-M-15/28 is shown as CTX-M-15, CTX-M-2/20/44/56/97 is shown as CTX-M-2, CMY 2/61 is shown as CMY-2, and SHV-12/129 is shown as SHV-12. Sequence references were provided by the Lahey Clinic (http://www.lahey.org/studies), and the following GenBank accession numbers were used for characterization: AB263754 for TEM-1b, AY883411 for TEM-52c, and AF126444 for TEM-52StPaul.