TABLE 3.
Genotypic ESBL characteristics of nWT isolates in fecal samples collected weeklya
| Dog | ESBL(s) detected (no. of isolates) at wk: |
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | |
| 2A | X | X | X | X | X | X | X | X | CMY-2 | NE (1) | CTX-M-3, NE (1) | ||||||
| 3A | X | X | X | X | X | X | CMY-2 (3) | X | NE (1) | CTX-M-1, CMY-2 | X | X | X | ||||
| 5A | X | X | X | X | X | X | NE (2) | X | X | X | X | X | X | ||||
| 5B | X | X | X | NE (3) | NE (1) | CMY-2 | X | CTX-M-1 | CTX-M-15 (3) | NE (1) | X | CTX-M-1 (3), TEM-52c | X | ||||
| 8A | X | X | X | X | X | X | X | X | X | X | X | ||||||
| 12A | X | X | CTX-M-1, SHV-12 (3) | CMY-2, NE (3) | X | X | CTX-M-1 (3) | CMY-2, TEM-35 | X | X | X | X | |||||
| 12B | X | X | X | NE (3) | NE (3) | CMY-2 | X | X | X | X | X | X | |||||
X, fecal sample without growth on MCC; NE, ESBL-negative isolates with non-wild-type susceptibility to cefotaxime. Dogs with the same numbers shared the same household. If a fecal sample produced >1 isolate containing similar genes, the number of isolates is shown in parentheses. Of isolates with combinations of resistance genes, only ESBL-type genes are shown. Gene type CTX-M-1/61 is shown as CTX-M-1, CTX-M-15/28 is shown as CTX-M-15, CTX-M-2/20/44/56/97 is shown as CTX-M-2, CMY 2/61 is shown as CMY-2, and SHV-12/129 is shown as SHV-12. Sequence references were provided by the Lahey Clinic (http://www.lahey.org/studies), and the following GenBank accession numbers were used for characterization: AB263754 for TEM-1b, AY883411 for TEM-52c, and AF126444 for TEM-52StPaul.