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Synthetic lethality assay for PBP1a/LpoA and PBP1b/LpoB. ΔmrcA/mrcA and ΔlpoA/lpoA represent the ΔmrcA and ΔlpoA strains, respectively, complemented with pHGC02 harboring a copy of the corresponding S. oneidensis genes in trans. Cells from the late logarithmic phase (OD600 ≈ 0.6) were used to prepare a decimal dilution series. Three microliters of each dilution was placed on an LB plate supplemented with or without 0.002% arabinose.
