TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli strains | ||
| DH5α | Host strain for plasmids | Lab stock |
| WM3064 | Donor strain for conjugation; ΔdapA | W. Metcalf, UIUC |
| S. oneidensis strains | ||
| MR-1 | Wild type | Lab stock |
| HG0280 | ΔmrcA strain derived from MR-1 | This study |
| HG0300 | ΔlpoA strain derived from MR-1 | This study |
| HG0633 | ΔmrcB strain derived from MR-1 | This study |
| HG0837 | ΔblaA strain derived from MR-1 | 39 |
| HG1060 | ΔlpoB strain derived from MR-1 | This study |
| HG3814 | ΔampG strain derived from MR-1 | 41 |
| HG0280-0837 | ΔmrcA ΔblaA strain derived from MR-1 | This study |
| HG0300-0837 | ΔlpoA ΔblaA strain derived from MR-1 | This study |
| HG0280-3814 | ΔmrcA ΔampG strain derived from MR-1 | This study |
| WT/PblaA-lacZ | Wild type harboring the PblaA-lacZ fusion | This study |
| Plasmids | ||
| pHGM01 | Apr Gmr Cmr; suicide vector | 42 |
| pHG101 | Promoterless vector for complementation | 43 |
| pHGC02 | pHG101 harboring the PBAD promoter | This study |
| pFAC | Mariner-based transposon vector | 44 |
| pHGT02 | pFAC removing the Apr gene | This study |
| pHGEI01-PblaA | pHGEI01 containing the blaA promoter | 41 |