TABLE 1.

Oligonucleotides used for the study of gene expression in this study

Species	Gene	Primer directiona	Primer sequence (5′ to 3′)	Amplicon size (bp)	Melting temp (°C)	Annealing temp (°C)
E. coli and K. pneumoniae	acrB	F	TTTGCGTGGGTGATCGC	192	54	50
		R	GTTATCGATACCGTTCATA		52
E. coli	acrF	F	TGGCCATTATYCTGATGA	179	50	48
		R	GTTATCGATACCGTTCATA		52
K. pneumoniae		F	TTCATCGACCGGTCTTCG	208	56	52
		R	AGATTGTCGATGCCGTTC		54
E. coli and K. pneumoniae	acrD	F	GTGCTGGCAATCCTGTT	181	52	50
		R	TATCGAGGCCGGTCATAT		54
E. coli	marA	F	TCATAGCATTTTGGACTGG	172	54	52
		R	TTGCGCGATTTCCGTCAT		54
K. pneumoniae		F	AGCGCTCCGGTTACTCTAA	171	58	56
		R	GCTGCGACTCGAAACCATA		58
E. coli	soxS	F	ATTGACCAGCCGCTTAACAT	197	58	54
		R	ACATAACCCAGGTCCATTG		56
K. pneumoniae		F	TAGTCGCCAGAAAGTCAGG	190	58
		R	GAGAAGGTTTGCTGCGAGA		56
K. pneumoniae	ramA	F	CTGCAACGGCTGTTTTTACA	180	58	56
		R	ATTGAAGGTCCGGGTGAAG		58
E. coli	gapA	F	GCTAACCTGAAATGGGACG	189	58	56
		R	GTCCTGGCCAGCATATTTG		58
K. pneumoniae	gapA	F	GAAAGGCGTTCTGGGTTAC	184	58
		R	GATGTGGGCAATCAGATCC		58

^aF, forward; R, reverse.