. 2015 May 14;59(6):3413–3423. doi: 10.1128/AAC.00187-15

TABLE 2.

Diversity of mutants and their biocide and antibiotic susceptibility

Species and biocide phenotype^a	Preconditioning agent^b	Biocide MIC (mg/liter)			Antibiotic MIC (mg/liter)^c							Mutant designation^d	No. of CFU by plate^e	No. of mutants
Species and biocide phenotype^a	Preconditioning agent^b	TRI	BKC	CHX	AMP	CAZ	CIP	ERY	GEN	CLO	TET	Mutant designation^d	No. of CFU by plate^e	No. of mutants
E. coli
Parental strain HEC30		0.06	16	8	≥256	0.25	0.023	16	1–1.5	6	3
TRI^r	TRI	4	16	2	≥256	0.13	0.032	16	0.75	3	3	Ec_TRI/TRI2	2	3
	CIP	1	32	8	≥256	0.75	0.75	16	1	12	0.75	Ec_CIP/CIP	7
	BKC	0.25	16	8	≥256	0.5	0.032	16	1.5	4–12	3	Ec_BKC/BKC2	1
TRI^r CHX^s	NE	1	16	4	≥256	0.38	0.023	16	1–1.5	6	2	Ec_NE/TRI1	UC	1
K. pneumoniae
Parental strain 39.11		0.5	16	32	32	0.38	0.047	16	1	2	3
TRI^r	AMP	2	32	64	64	0.75	0.094	16	1	128	4	Kpn_AMP/TRI2	7	16
	NE	2	32	64	128	0.5	0.5	16	1	>256	12	Kpn_NE/CIP	126
	TRI	2	32	64	≥256	0.5	0.5	16	1.5	>256	8	Kpn_TRI/CIP	177
	CHX	2	32	64	≥256	0.38	0.38	32	0.75	>256	8	Kpn_CHX/CIP	59
	BKC	2	32	64	≥256	1	0.38	96	0.75	64	>128	Kpn_BKC/CIP	53
	SHC	2	32	64	128–192	0.75	0.5	32–48	1.5	>256	16–24	Kpn_SHC/CIP	31
	NE	2	32	32	32	0.5	0.38	16	1	>256	4	Kpn_NE/TRI1	UC
	TRI	2	32	32	64	0.5	0.38	12	1	128	6	Kpn_TRI/TRI2	272
	BKC	2	32	32	48	0.5	0.38	12	1	128	4	Kpn_BKC/TRI2	12
	CHX	2	32	32	32	0.5	0.5	16	1	>256	4	Kpn_CHX/TRI2	6
	CIP	2	32	32	32	0.38	0.5	32	1	>256	24–32	Kpn_CIP/TRI2	20
	TRI	2	32	32	≥256	0.38	0.5	16	0.5	>256	16–24	Kpn_TRI/BKC1	UC
	BKC	2	32	32	≥256	1.5	0.38	16	0.5	192	32	Kpn_BKC/BKC1	UC
	CIP	2	32	32	≥256	1.5	0.75	>256	0.19	96	12	Kpn_CIP/BKC2	11
	AMP	2	32	32	≥256	0.5	0.5	16	0.5	>256	8	Kpn_AMP/CIP	152
	CIP	2	32	32	192	0.5	0.38	16	1	>256	12	Kpn_NE/CIP	126
TRI^hs	NE	0.12	8	32	48	0.25	0.094	16	1.5	3	2	Kpn_NE/CHX2	3	2
	CIP	0.12	16	16	32	0.25–0.38	0.094	16	1	4	1.5	Kpn_CIP/CHX2	UC

TRI, triclosan; CHX, chlorhexidine.

CIP, ciprofloxacin; BKC, benzalkonium chloride; NE, no exposure; AMP, ampicillin; SHC, sodium hypochlorite.

CAZ, ceftazidime; ERY, erythromycin; GEN, gentamicin; CLO, chloramphenicol; TET, tetracycline.

When the selecting agent was TRI, the numbers in the nomenclature of mutants refer to the concentrations of the compounds in the plates as follows: 1 = 1 mg/liter and 2 = 1.5 mg/liter. When the selecting agent was CHX, the concentration in the plate was as follows: 1 = 64 mg/liter or 2 = 128 mg/liter. In the case of BKC, the numbers 1 to 3 refer to the concentrations in plates as follows: 1 = 32 mg/liter, 2 = 64 mg/liter, and 3 = 128 mg/liter. The concentration of CIP was 0.12 mg/liter in all plates.

UC, uncountable.