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The binding affinity of peptides to the LPS from P. aeruginosa ATCC 27316 (25 μg/ml) was determined by the BODIPY-TR-cadaverine displacement method as described in Materials and Methods. Fluorescence intensity was monitored at an excitation wavelength of 580 nm and an emission wavelength of 620 nm. The graphs were derived from average values of three independent trials. ∗, P < 0.01; ∗∗, P < 0.001 (compared to results with polymyxin B at the same concentration). AU, arbitrary units.
