FIG 3.

(A) Temperature sensitivity of C. albicans mutants exhibiting CRS-MIS (34.80-C2 and SN95F1F12) at 42°C and its reversion following reconstitution of wild-type TSC13 (34.80-C2r36 and 34.80-C2r37) or FEN12 (SN95F1F12r11 and SN95F1F12r12). Approximately 50 or 500 cells were spotted in duplicate on YPD agar plates and incubated at 35°C or 42°C for 2 days. (B) PCR confirmation of FEN12 restoration in SN95F1F12r11 and SN95F1F12r12. Genomic DNA was amplified with the indicated primer pairs and analyzed by gel electrophoresis. Lane L, DNA ladder (kbp); lane 1, SN95 parent; lane 2, SN95F1; lane 3, SN95F12; lane 4, SN95F1F12; lane 5; SN95F1F12r11; lane 6, SN95F1F12r12. Expected size of CaFEN12u403uF-CaFEN12c558R product = 961 bp; expected size of CaFEN12c329F-CaFEN12d110R product = 671 bp.