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Nitrite induces resistance to aminoglycosides within biofilms. PAO1 biofilms were grown on the surface of CFBE-wt cells for 6 h and then treated with nitrite (A) or nitrite and tobramycin (B) for 90 min. Brackets indicate differences with P values of <0.05 by one-way ANOVA followed by pairwise comparison testing. (C) The zone of inhibition is unchanged in biofilm bacteria exposed to nitrite compared to unexposed bacteria (n = 3; representative images are shown).
