FIG 1.

Schematic gene map showing the erm(45)-containing island and flanking region of S. fleurettii JW205 (ENA accession number LN680996), as well as the chromosomal glutamine amidotransferase gene (guaA) region of erm(45)-negative strain JW404. Gray areas represent regions showing high similarity at nucleotide level (>98%). Arrows represent position and orientation of open reading frames (ORFs). The erm(45) and its leader peptide (lp) gene are shown in green. The 11-bp (GAGTGGGAATA) chromosomal hot spot situated within the 3′ end of guaA is abbreviated as att_C. This attachment site is illustrated as att_L and att_R at each extremity of the island. The IS431 transposase gene (tnp) illustrated in yellow is flanked by 16-bp inverted repeats (IR) (GGTTCTGTTGCAAAGT). Other putative genes and functions: KilA-N, KilA-N domain-carrying putative DNA-binding protein; c1/cro, repressors; Bro-N, Bro-N domain-carrying putative DNA-binding protein; lysR, transcriptional regulator; int, integrase; prim, primase. Color code: S. fleurettii core genome, orange; erm(45)-carrying island genes related to SaPI and bacteriophage genomes, blue; antibiotic resistance, green; no known function, significant similarity to proteins in the database used, or any known protein signatures, gray. Primers used to detect circular forms of the erm(45)-carrying island are indicated by small black arrows. The figure was generated using the program Easyfig (25).