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Immunoreactivity of O25b-specific humanized MAbs with purified LPS. Purified LPS samples from E. coli serotypes O25b, O25a, and O55 (control) were separated and blotted onto PVDF membranes that were reacted with the indicated MAbs. The inner-core-specific cross-reactive WN-1 222-5 MAb reacting with all LPS types was used as a positive control.
