FIG 6.

In vitro bactericidal efficacy of aglycosylated MAbs. Bactericidal activities of three aglycosylated MAbs were determined simultaneously with their glycosylated counterparts. Bactericidal activity is expressed as percent killing, i.e., CFU recovered in test groups relative to the control group (irrelevant MAb control) following 3 h of incubation in 50% depleted human serum. (A) MAb 3E9-11; (B) MAb 4D5-02; (C) MAb 2A7-01. Black bars, wild-type MAbs; gray bars, Fc mutated to remove glycosylation. Means with range for 2 independent assays are shown.