Skip to main content
. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: J Exp Zool A Ecol Genet Physiol. 2014 Oct 28;321(10):595–602. doi: 10.1002/jez.1891

Figure 1.

Figure 1

Antibody validation of pDARPP-32 on nucleus accumbens of sectioned brain tissue. Immunohistochemistry was conducted on cryosectioned leopard gecko (Eublepharis macularius) brain tissue using a rabbit polyconal antibody against pDARPP-32, and DAB staining was used to visualize pDARPP-32 immunoreactive cells. Bars indicate 50 μm. (A) Without pDARPP-32 antibody, no cells were detected. (B) pDARPP-32 neuronal staining was detected when tissue was incubated with antibody. (C) pDARPP-32 neuronal staining was not detected when antibody was preadsorbed with 5× pDARPP-32 peptide prior to incubation.