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. 2015 Mar 13;290(20):12463–12473. doi: 10.1074/jbc.M114.629519

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Inhibition of NCX1 activity increases intracellular calcium, activates ERK1/2, and increases rate of migration in MDCK cells. Plots depicting the intracellular calcium response of MDCK cells loaded with 10 μm Fluo-4 AM to 10 μm KB-R7943 with (A) or without (B) calcium in the medium. The arrow indicates the time of KB-R7943 addition. Representative plots from three independent experiments (each with greater than 25 cells) are shown. Note the increase in intracellular calcium concentration upon the addition of 10 μm KB-R7943 (arrow). C, immunoblots show the phosphorylation status of ERK1/2 and Akt with β-actin as loading control in MDCK, β-KD, and MDCK cells treated with 10 μm KB-R7943. Representative blots from three independent experiments are shown. D, the graph denotes the average rate of migration of MDCK cells with or without 10 μm KB-R7943 treatment as measured by wound healing assay from three independent experiments performed in triplicate. Error bars denote S.E., and the asterisk indicates statistical significance (*, p < 0.05).