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. 2015 Mar 13;290(20):12463–12473. doi: 10.1074/jbc.M114.629519

FIGURE 7.

FIGURE 7.

NCX1 inhibition by KB-R7943 increases ERK1/2 and MLC phosphorylation and rate of migration in renal epithelial cells in a PI3K-dependent manner. A, representative images of the wound in LLC-PK1 cells treated with different concentrations of KB-R7943 at 0 h and after 16 h. Scale bar = 100 μm. B, the graph represents the mean rate of migration calculated from three independent experiments in triplicate. Error bars denote S.E., and the asterisks indicate statistical significance (**, p < 0.005). C, corresponding immunoblots show the phosphorylation status of ERK1/2 and Akt with total ERK1/2 as the loading control in LLC-PK1 cells treated with the indicated concentrations of KB-R7943 for 16 h. D, the graph represents the average rate of migration of HREpiC cells treated with 10 μm KB-R7943 in the presence or absence of 10 μm LY294002 or 10 μm PD98059 for 10 h. Error bars denote S.E., and the asterisks indicate statistical significance (**, p < 0.005). E, corresponding immunoblots show the phosphorylation status of ERK1/2 and MLC with β-actin as loading control in HREpiC cells. Quantification from three independent experiments expressed as -fold change normalized to β-actin loading control are indicated below the blot. F, the graph shows the average rate of migration in β-KD cells treated with DMSO, 5 μm ML-7, or 5 μm Y-27632 for 16 h by ECIS wound healing assay from three independent experiments. Error bars denote S.E. (**, p < 0.005).