FIGURE 3.

Formation of UreE-UreG complex. UreE and UreG (40 μm for each) samples were incubated with 1.5 molar eq of metal ions (Ni²⁺/Zn²⁺/Mg²⁺) with or without supplementation of guanine nucleotides (GDP or GTP) and loaded onto an analytic column. A, the formation of UreE-UreG complex in the absence of Mg²⁺. Profiles of free UreE and UreG are shown for comparison (lines 1 and 2). B, the formation of UreE-UreG complex in the presence of Mg²⁺. Note that 2E-2G complex is formed only in the presence of both GTP and Mg²⁺ (lines 5, 7, and 9).