TABLE 1.

Primers used for PCR reactions (restriction sites or mutated sites are bolded)

The abbreviations used are: for, forward; rev, reverse.

For pET28a-ureG
UreG(NdeI)-for	GGAATTCCATATGGTAAAAATTGGAGTTTGTG
UreG(EcoRI)-rev	CGGAATTCCTAATCTTCCAATAAAGCGTTGC
For pET28a-ureG(C66A)
UreG(C66A)-for	CAGGAGGCGCTCCGCACACGGCTATTAG
UreG(C66A)-rev	TTTCTACGCCAATGATCCTCTCTCGTGG
For pET28a-ureG(H68A)
UreG(H68A)-for	CAGGAGGCTGTCCGGCCACGGCTATTAG
UreG(C66A)-rev
For pET28a-ureE(N100A)
UreE(N100A)-for	CTATGAAATAGGAGCGCGCCATGCG
UreE(N100A)-rev	CATATTTTCGCTACTTCTGCCACGC
For pET28a-ureE(R101A)
UreE(R101A)-for	CTATGAAATAGGAAACGCCCATGCGG
UreE(N100A)-rev
For pET28a-ureE(H102A)
UreE(H102A)-for	CTATGAAATAGGAAACCGCGCTGCGG
UreE(N100A)-rev
For pET28a-ureE(H152A)
UreE(H152A)-for	AACCGTGAGCATGCCCGCGAGTGAGC
UreE(H152A)-rev	AAGCGTTCTTTGGAATCCAATTTTGAAC
For pET-UreA2H
UreA(NdeI)-for	GGAATTCCATATGAAACTCACCCCAAAAGAGTTAG
UreH(EcoRI)-rev	CGGAATTCTCAAACCTTTTGCGTGGTGGTTTGC
For pET-UreA2HΔG
ΔUreG-for	TGATGAACACTTACGCTCAAGAATCCAAG
ΔUreG-rev	TCAAGACATATAAAGGCGCGAGTATAAAC