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. 2015 Mar 18;290(20):12497–12503. doi: 10.1074/jbc.M114.635540

FIGURE 3.

FIGURE 3.

Acetylation increases ENaC current. A, superimposed representative short-circuit current traces in FRT epithelia transfected with α-, β-, and γENaC (0.5 μg of each) and treated with TSA (50 nm, black) or vehicle (ethanol, gray) for 24 h. Amiloride (10 μm) was added to the apical bathing solution, as indicated by the black bar. B, quantification of amiloride-sensitive short-circuit currents (relative to −TSA groups) in FRT epithelia transfected with wild type ENaC or cytoplasmic K-R mutant ENaC (K-R) (mean ± S.E., n = 12–14; *, p < 0.0001). Non-normalized currents in the absence of TSA were: wild type, 2.82 ± 0.2 μA/cm2; K-R, 0.73 ± 0.03 μA/cm2. C, representative short-circuit current traces in mpkCCD epithelia treated with TSA (50 nm, black) or vehicle (ethanol, gray) for 24 h. D, quantification of amiloride-sensitive short-circuit currents in mpkCCD epithelia (relative to −TSA group) (mean ± S.E., n = 14; *, p < 0.009).