FIGURE 1.

Subcellular localization of Kv1.3 protein in cancer cells and human brain tissues. Western blot (WB) analysis of Kv1.3 shows labeling within the nuclear fraction of three cancer cell lines (MCF7, A549, and SNU-484) (n = 4) (A) and human brain tissue extracts (n = 3) (B). The specificity of the fractionation process was confirmed using specific antibodies including: ATP5a, GRP78 BiP, and Na,K-ATPase, three membrane markers, and lamin A, a marker for the nucleus. Lanes C, cytosol; lanes M, membrane; lanes N, nucleus.