FIGURE 2.
HO-1 was downstream of NHE1 in K562R cells. A, pHi value was reduced with increasing concentration of cariporide. The whiskers above and below the plots designate the 95th and 5th percentiles, respectively. B and C, cells were incubated with the indicated concentration of cariporide for 2 h. Protein in cell lysates was analyzed by Western blot using HO-1, p-Ser, and NHE1 specific antibodies. Con, control. D and E, cells were harvested at various time intervals. HO-1, p-Ser, and NHE1 protein expressions levels were detected. F, NHE1 was silenced by siRNA, and then HO-1 and p-Ser were detected. G, K562R cells were treated by IM alone and in combination with cariporide or ZnPP IX. Cells viability was detected by CCK-8 assay and compared with that of K562 cells treated by IM as well. The curve and whisker plots were made to assess mRNA and protein expressions. ** indicates p < 0.01, and * indicates p < 0.05.