FIGURE 2.

EDD coimmunoprecipitates with SAC- and APC-associated components. A and B, HeLa (A) and HCT116 (B) cells were treated with either EDD or scramble control siRNAs. Following siRNA treatment, lysates were immunoprecipitated with CDC20, BUBR1, or IgG control (Ctrl) antibodies, and coimmunoprecipitating proteins were analyzed by SDS-PAGE and Western blotting with the indicated antibodies. Ponceau Red-stained membranes are included as a loading control for the lysate inputs. Images are representative of two independent experiments.