FIGURE 4.

EDD siRNA abrogates nocodazole-mediated G₂/M accumulation and increases expression of CDC20, phospho-APC3, and BUBR1. HeLa cells were treated with the indicated siRNAs, treated with nocodazole (A–C, E, and F) or Taxol (D) for 18 h, released, and imaged by brightfield microscopy (A) and analyzed by propidium iodide staining and FACS analysis (B and D, respectively). siRNA efficiency (C) and siRNA-treated cells released from Noc for the indicated times post-release (E) were analyzed by SDS-PAGE and Western blotting with the indicated antibodies. F, density quantification of bands in E, with the values expressed relative to scrambled siRNA t0 values. The data in B and D were analyzed using one-way analysis of variance and Tukey's multiple comparison test, which revealed all G₁ or G₂/M comparisons to be significantly different.