FIGURE 6.
EDD siRNA does not mediate G1 arrest but does increase p21 and γH2AX levels. A and B, HeLa cells were treated with scramble control (Con), EDD, BUBR1, or combined (Combo) siRNAs for 48 h and then analyzed by γH2AX (A) or propidium iodide FACS analysis (B). A, values represent the -fold increase over basal γH2AX levels (47) detected in scramble control cells. A, data were analyzed using unpaired Student's t test with the p value indicated. B, data were analyzed using one-way analysis of variance and Tukey's multiple comparison test, which revealed significant differences between the control and both BUBR1- and Combo siRNA-treated samples. A and B, errors bars represent mean ± S.E. from three experiments. C, SDS-PAGE and Western blotting with the indicated antibodies confirmed the EDD siRNA-mediated knockdown and induction of p21.