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. 2015 Mar 18;290(20):12603–12613. doi: 10.1074/jbc.M115.636506

FIGURE 2.

FIGURE 2.

Depletion of macrophages after clodronate treatment. A, liposome-clodronate (1 mg in 0.2 ml, intravenously) or vehicle (phosphate-buffered saline) was administered twice to mice (1 day prior to PSL and 4 days after PSL), and PMs were collected on day 7 after PSL. The PM population was evaluated by flow cytometry using fluorescent monoclonal antibodies (Abs) for macrophages (FITC-conjugated anti-CD11b Ab and phycoerythrin (PE)-conjugated anti-F4/80 Ab). B, Clophosome-ATM (0.1 ml, i.v.; FormuMax Scientific, Palo Alto, CA) or vehicle (PBS) were administered once (1 day before PSL) to mice, and PMs were collected on day 4 after PSL. The PM population was quantified using FITC-conjugated anti-CD14 Ab and PE-conjugated anti-F4/80 Ab. C and D, histogram analysis shows the F4/80+ macrophage counts after administration of liposome-clodronate or Clophosome-ATM.