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. 2015 Mar 18;290(20):12603–12613. doi: 10.1074/jbc.M115.636506

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Expression of GITRL and GITR in the SCN after PSL and murine PMs. A, the time course of GITRL (Gitrl) and GITR (Gitr) induction in the SCN on days 3, 7, and 14 after PSL. Gene expression levels measured by reverse transcription polymerase chain reaction were normalized to levels of glyceraldehyde-3-phosphate dehydrogenase (Gapdh) expression and analyzed using ImageJ software (National Institutes of Health). Data indicate mean ± S.E. for groups of 12 mice; ***, p < 0.001, and **, p < 0.01, compared with levels before PSL. B, the gene expression levels of Gitrl, Gitr, and Gapdh in naive murine PMs and in the SCN after PSL or sham operation on day 7. C and D, localization of GITRL and GITR in the injured SCN. Representative micrographs of sections stained using immunohistochemistry show the SCN on day 7 after PSL or sham operation. Co-localization of GITRL and macrophage (F4/80) or T cell (CD4) markers is shown. Green, GITRL or GITR; red, F4/80 or CD4; yellow, co-localized expression. Scale bar = 20 μm.