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. 2015 Mar 18;290(20):12603–12613. doi: 10.1074/jbc.M115.636506

FIGURE 9.

FIGURE 9.

Therapeutic effects of anti-GITRL antibody on PSL-induced thermal hyperalgesia and tactile allodynia. Anti-GITRL antibody (GITRL Ab; 10 or 100 ng) or control immunoglobulin G (IgG) was perineurally administered (10 μl) five times (on days 0, 3, 6, 9, and 12 after PSL). Thermal hyperalgesia and tactile allodynia were assessed by the Hargreaves test or the von Frey test, respectively. A and B, a time course of the suppressive effect of GITRL Ab on PSL-induced thermal hyperalgesia and tactile allodynia. C and D, dose-dependent effects of GITRL Ab on PSL-induced thermal hyperalgesia and tactile allodynia. IgG, control IgG; ipsi, ipsilateral hind paw of the PSL-operated side; contra, contralateral hind paw of the PSL-operated side. Data shown indicate mean ± S.E. of groups of five mice; ***, p < 0.001 and **, p < 0.01 versus IgG contra; ###, p < 0.001, and ##, p < 0.01 versus IgG ipsi. E, effects of GITRL Ab on the 50% withdrawal threshold after PSL. GITRL Ab was perineurally injected according to the administration schedule. Data shown indicate mean ± S.E. of groups of 5–8 mice; ***, p < 0.001 and **, p < 0.01 versus sham IgG; ##, p < 0.01, and #, p < 0.05 versus PSL IgG.